BIOE 800 Bioengineering Colloquium
All seminars take place from 4:00 p.m. - 5:00 p.m. in 3150 Learned Hall, unless otherwise specified.
Stevin Gehrke, Ph.D.
New FALL 2013 students ONLY. This is a colloquium review session.
Candan Tamerler, Ph.D.
Wesley G. Cramer Associate Professor, Mechanical Engineering, University of Kansas
Nature continues to provide the inspiration for engineering structural and processing design criteria of materials and systems to perform life`s functions. With a growing understanding of the molecular processes involved, bio-inspired strategies are increasingly explored to obtain hierarchical structures with unique properties and bio-enabled materials. The challenges in these strategies include controlling the self-organization at a molecular level and thus provide control over the biological and inorganic interfaces under the environmentally benign and biologically compatible conditions. In biological systems, proteins conduct various functions through their unique molecular recognition, self-assembly and templating properties. In addition to their role in biomineralization, proteins perform a wide spectrum of additional functions ranging from catalysis to self-repair. Our inspiration has been to decode the foundations in biology`s highly organized and multifunctional structures and, thereby, design advanced materials using biological principles by engineering well-defined peptide/protein based inorganic interfaces. Building upon the modularity of protein domains, we design single to multifunctional chimeric peptides or recombinant proteins With an extensive array of multifunctional molecular units, we tackle different areas including designing organic-inorganic interfaces, biological surface functionalization, multifunctional protein/peptide based hybrid nanoprobes for sensing and targeting, peptide enabled mineralization on tissues and implant surfaces as well as bionano fabrication for material synthesis and processing.
Omar Aljitawi, M.D.
Internal Medicine; Department of Hematology/Oncology, Blood and Marrow Transplantation
KU Medical Center
The colloquium will focus on 3D in vitro microenvironment related to the clinical applications that Dr. Aljitawi’s laboratory has been focusing on. He will discuss the interaction of stem cells with their microenvironment and how this can be utilized in expanding umbilical cord blood stem cells, in improving umbilical cord blood homing post- transplant, and in developing a three dimensional leukemia and myeloma in vitro models for chemotherapy testing. Dr. Aljitawi will address his studies regarding Wharton's jelly matrix as a scaffolding material for tissue regenerative applications like bone and cartilage regeneration.
Lorin Maletsky, Ph.D.
Associate Professor, Associate Department Chair of Mechanical Engineering, University of Kansas
Dr. Maletsky’s research area is machine design and biomechanics, specifically the experimental testing of cadaveric joints using custom-designed physiological loading equipment. He has had funding from the orthopedic industry, the National Science Foundation, and the Center for Disease Control. Graduates from his laboratory hold positions in a number of the world’s largest orthopedic companies, as well as in academia and in other industries. He is a member of the Bioengineering Division of ASME and currently chairs the Design, Dynamics, and Rehabilitation Technical Committee. Dr. Maletsky is also a member of the Orthopedic Research Society and the American Society for Engineering Education
James Piret, Sc.D.
Professor, Chemical & Biological Engineering, University of British Columbia
Cell based therapies have great potential to one day provide treatments for major diseases from cancer to cardiac and neurodegenerative diseases. There is a growing need to efficiently develop and assess the quality of cell manufacturing technologies. Raman microspectroscopy offers a non-invasive and label-free approach to monitor the chemical composition of living cells. Spectral markers, such as the ratio of nucleic acid to tyrosine associated band intensities, can discriminate between pluripotent stem cells and their differentiated progeny. This can provide a rapid, noninvasive approach to distinguish stem cells from differentiated cells using changes in cell composition. Cellular responses to nutrient deprivation also can be analyzed by Raman microspectroscopy. For example, phospholipid-associated Raman band intensity increase upon the autophagic response to glutamine limitation. Thus, Raman spectral indicators can be used to non-destructively monitor cell physiological changes in response to the culture environment as well as stem cell differentiation to the desired product.
Gregory Cooksey, Ph.D.
National Institute of Standards and Technology
Following the discovery that adult cells can be reprogrammed to a pluripotent state, pluripotent stem cells (PSCs) are being developed at a rapid pace for therapeutic and disease-related applications. With potential differences in cell line quality and changes in colonies with time, it is important to have methods that can discriminate amongst different cell types. Pluripotent antibodies are common tools used to assess the day-to-day quality of PSC cultures. However, a lack of benchmarking techniques makes it difficult for two laboratories to compare measurements. To address this challenge, we have developed a robust cell-labeling strategy that allows antibody stains to be normalized to total cellular protein. I will describe the ratiometric strategy that permits measurement normalization across different instruments, and I will demonstrate how we have used the method as a specification for induced PSC (iPSC) colonies over several weeks of culture. Finally, I will show how the method enables systematic evaluation of culture conditions and staining protocols with pluripotent antibody markers on iPSCs.
In the latter part of the talk, I will discuss my work using microfluidic devices to control and measure complex spatiotemporal behavior in arrays of cells. In particular, I will describe systems that my collaborators and I have developed to assess cell toxicity with chemical gradients and to probe the olfactory system. I will also touch on some new work using simple fabrication techniques to make novel three-dimensional microfluidic chips.
Greg Cooksey is a bioengineer at the National Institute of Standards and Technology (NIST) in Gaithersburg, MD. His research efforts focus on the development of methods to improve the measurements of living cells and systems. In particular, his current work is focused on improving comparability of antibody-based measurements in the determination of stem cell identity. He has worked extensively to design microfluidic devices and utilize microscopy to study stem cells, cytotoxicity, olfaction, and migration. Dr. Cooksey earned his Ph.D. in bioengineering at the University of Washington (UW).
Dr. Cooksey graduated from the University of Kansas department of electrical engineering in 1999. At KU, he worked with Dr. Robert Dunn in the Chemistry Department to develop near-field scanning optical microscopy (NSOM) probes for use in biological imaging. He was a Barry M. Goldwater Scholar, and he received a Whitaker Graduate Fellowship in Bioengineering and National Research Council Postdoctoral Research Associateship. Dr. Cooksey grew up in Quinter, Kansas, which is about 4 hours west of Lawrence on I-70.
Special Session: Judging Biomedical Engineering Posters from CPE 656/756
Please meet in 3150 Learned Hall at 4:00 on Friday December 6th for information and instructions regarding how to judge the poster session. You will then continue on to the Eaton Atrium for judging.
During the poster session BIOE 800 students will be judging CPE 656 undergraduate students. The posters will display individual CPE 656/756 student semester projects.
Stevin Gehrke, Ph.D.
New SPRING 2014 students ONLY. This is a colloquium review session.
Anil Misra, Ph.D.
Professor; Civil, Environmental and Architectural Engineering; University of Kansas
Synopsis coming soon
Brendan Harley, Sc.D.
Assistant Professor; Chemical and Biomolecular Engineering, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
The extracellular matrix (ECM) is a complex organization of structural proteins such as collagens and proteoglycans. Understanding that the ECM is dynamic and often spatially patterned or heterogeneous over the length-scale of traditional biomaterials, we are developing instructive biomaterials that present microenvironmental cues in spatially and temporally defined manners. I will describe development of a collagen biomaterial to address critical barriers preventing regeneration of orthopedic insertions such as the osteotendinous (tendon-bone) junction. Here, replicating spatial gradients in mineral content and matrix anisotropy across a single biomaterial construct enables us to drive mesenchymal stem cell (MSC) differentiation down osteotendinous lineages in a spatially-selective manner. Further, we have created bioinspired core-shell structures (e.g., porcupine quills) in order to balance bioactivity and mechanical competence concerns. I will subsequently describe a microfluidic forming technique to create libraries of optically-translucent hydrogels containing overlapping patterns of cell, matrix, and biomolecule cues. We are using this ‘tissue biochip’ platform to dissect the coordinated impact of spatially-organized cell and matrix signals on(1) niche-mediated regulation of hematopoietic stem cell fate; and (2) the malignancy and therapeutic response of human glioblastoma multiforme cells. I will show how these biomaterial platforms can be used as rheostats to regulate critical cellular processes such as stem cell self-renewal vs. differentiation; tissue regeneration and vascularization; and the etiology and malignancy of cancer.