Assistant Professor of Chemistry
Analytical Chemistry, Micro/Nanofluidics, Single Cell Analysis, Genetic Analysis, Proteomics, Cancer Biology and Diagnostics, Systems Biology, Nanobiotechnology
Quantitative analysis of dynamic and heterogeneous biological processes is key to deciphering complex biological systems, such as cancer. Drawing on microfluidics, analytical chemistry, molecular/cell biology, bioengineering, and material science, our research is dedicated to advancing quantitative biology and personalized medicine through technology innovation and translational research. We currently focus on three main areas:
Wang, T; Zhang, M; Dreher, D; Zeng, Y “Ultrasensitive Microfluidic Solid-Phase ELISA Using an Actuatable Microwell-Patterned PDMS Chip”, Lab on a Chip, 2013, 13, 4190-4197. (Selected as a Lab on a Chip HOT Article, top 10%)
Shuga, J;†Zeng, Y;† Novak, R; Lan, Q; Tang, X; Rothman, N; Li, L; Hubbard, A; Zhang, L; Mathies, RA; Smith, MT “Single Molecule Quantitation and Sequencing of Rare Translocations Using Microfluidic Nested Digital PCR”, Nucl. Acids Res. 2013, 41 (16): e159 (†Equal contribution)
Zeng, Y; Wang, T “Quantitative Microfluidic Biomolecular Analysis for Systems Biology and Medicine” Analytical and Bioanalytical Chemistry, 2013, 405, 5743-5758.
Y. Zeng,* M. Shin, T. Wang, “Programmable Active Microfluidic Droplet Generation Enabled by Integrated Pneumatic Micropumps”, Lab on a Chip, 2013, 13, 267-273. (*Corresponding author)
R. Novak,* Y. Zeng,* J. Shuga, G. Venugopalan, D. Fletcher, L. Zhang, M. T. Smith, R. A. Mathies, “Single Cell Multiplex Gene Detection and Sequencing Using Microfluidically-Generated Agarose Emulsions”, Angew. Chem. Int. Ed., 2011, 50, 390-395. (*Equal contribution)
- Selected as a “Hot Paper” by the Editors
- Highlighted in the frontispiece.
E. C. Jensen,* Y. Zeng,* J. K. Kim, R. A. Mathies, “Microvalve Enabled Digital Microfluidic Systems for High Performance Biochemical and Genetic Analysis”, Journal of Association for Lab Automation, 2010, 15, 455-463. (*Equal contribution)
J. Shuga, Y. Zeng, R. Novak, R. A. Mathies, P. Hainaut, M. T. Smith, “Selected Technologies for Measuring Acquired Genetic Damage in Humans”, Environmental and Molecular Mutagenesis, 2010, 51, 851-870. (Invited review)
Y. Zeng, R. Novak, J. Shuga, M. T. Smith, R. A. Mathies, “High-Performance Single Cell Genetic Analysis Using Microfluidic Emulsion Generator Arrays”, Anal. Chem., 2010, 82, 3183-3190.
- Cover paper;
- News Focus: “The power of one” Science, 2011, 331, 24-26;
- Technology Feature: “Clever PCR: more genotyping, smaller volumes” Nature Methods, 2010, 7, 351-355;
- News: “Detecting extremely rare mutants with single-cell PCR technology” BioTechniques, 2010, April 27.
M. He, J. Bao, Y. Zeng, D. J. Harrison, “Parameters Governing Flow Properties of Porous Monoliths Photopatterned within Microfluidic Channels”, Electrophoresis, 2010, 31, 2422-2428.
Y. Zeng, M. He, D. J. Harrison, “Microfluidic Self-Patterning of Large-Scale Crystalline Nanoarrays for High-Throughput Continuous DNA Fractionation”, Angew. Chem. Int. Ed. 2008, 47, 6388–6391.
- Highlighted in “Another brick in the wall”, www.separationsnow.com5, 2010.